abstract：:The supply of substrates to the respiratory chain as well as of other metabolites (e.g. ATP) into inner compartments of mitochondria is crucial to preprotein import into these organelles. Transport of the compounds across the outer mitochondrial membrane is enabled by mitochondrial porin, also known as the voltage-dep...

journal_title：Acta biochimica Polonica

authors： Szczechowicz A,Hryniewiecka L,Kmita H

更新日期：2001-01-01 00:00:00

abstract：:Calretinin (CR) is a neuronal EF-hand protein previously characterized as a calcium (micromolar affinity) binding protein. CR-containing neurons are spared in some neurodegenerative diseases, although it is as yet unconfirmed how CR plays an active role in this protection. Higher levels of some metal cations (e.g. cop...

journal_title：Acta biochimica Polonica

authors： Groves P,Palczewska M

更新日期：2001-01-01 00:00:00

abstract：:Dendrimers are a new class of polymeric materials. They are highly branched, monodisperse macromolecules. The structure of these materials has a great impact on their physical and chemical properties. As a result of their unique behaviour dendrimers are suitable for a wide range of biomedical and industrial applicatio...

journal_title：Acta biochimica Polonica

authors： Klajnert B,Bryszewska M

更新日期：2001-01-01 00:00:00

abstract：:We report the synthesis, photochemical and photophysical properties and preliminary studies on biological effect of a new tritolylporphyrin dimer (T-D). Absorption and emission properties of T-D suggest its possible use in photodynamic therapy. T-D is capable of singlet oxygen production with 0.8 quantum yield. It als...

journal_title：Acta biochimica Polonica

authors： Drzewiecka A,Urbańska K,Matuszak Z,Pineiro M,Arnaut LG,Habdas J,Ratuszna A,Stochel G

更新日期：2001-01-01 00:00:00

abstract：:The object of this paper is to review briefly the studies on the interaction of red blood cell membrane skeletal proteins and their non-erythroid analogues with lipids in model systems as well as in natural membranes. An important question to be addressed is the physiological significance and possible regulatory molec...

journal_title：Acta biochimica Polonica

authors： Sikorski AF,Hanus-Lorenz B,Jezierski A,Dluzewski AR

更新日期：2000-01-01 00:00:00

abstract：:There are many diseases related to ion channels. Mutations in muscle voltage-gated sodium, potassium, calcium and chloride channels, and acetylcholine-gated channel may lead to such physiological disorders as hyper- and hypokalemic periodic paralysis, myotonias, long QT syndrome, Brugada syndrome, malignant hypertherm...

journal_title：Acta biochimica Polonica

authors： Dworakowska B,Dołowy K

更新日期：2000-01-01 00:00:00

abstract：:Chemical deglycosylation (dg) of sturgeon Acipenser gueldenstaedti Br. (alphaGTH) resulted in the loss of 83% of its initial carbohydrate content. It altered also recombinant dg alphaGTH + betaGTH dimer molecule, reducing its immunoreactivity by 30%, and fully blocking the hormonal function. CD spectroscopy showed tha...

journal_title：Acta biochimica Polonica

authors： Zenkevics H,Vose V,Vosekalne I,Bũcena A

更新日期：2000-01-01 00:00:00

abstract：:Lanthanides, also called rare-earth elements, are an interesting group of 15 chemically active, mainly trivalent, f-electronic, silvery-white metals. In fact, lanthanides are not as rare as the name implies, except for promethium, a radioactive artificial element not found in nature. The mean concentrations of lanthan...

journal_title：Acta biochimica Polonica

authors： Pałasz A,Czekaj P

更新日期：2000-01-01 00:00:00

abstract：:In contrast to most other eukaryotic organisms, yeast can survive without respiration. This ability has been exploited to investigate nuclear genes required for expression of mitochondrial DNA. Availability of complete Saccharomyces cerevisiae genomic sequence has provided additional help in detailed molecular analysi...

journal_title：Acta biochimica Polonica

authors： Chacińska A,Boguta M

更新日期：2000-01-01 00:00:00

abstract：:Syntheses of biomimetic low-molecular weight poly-(R)-3-hydroxybutanoate mediated by three types of supramolecular catalysts are presented. The utility of these synthetic polyesters for preparation of artificial channels in phospholipid bilayers capable of sodium and calcium ion transport across cell membranes, is dis...

journal_title：Acta biochimica Polonica

authors： Jedliński Z,Kurcok P,Adamus G,Juzwa M

更新日期：2000-01-01 00:00:00

abstract：:(E)-2'-deoxy-2'-(fluoromethylene)-cytidine (FMdC), a deoxycytidine analog displaying a very high toxicity toward a variety of solid tumor cell lines and xenografts, is activated intracellularly by deoxycytidine kinase (dCK). We have compared cytotoxicity of FMdC towards a human promyeolocytic leukemia line HL-60 and a...

journal_title：Acta biochimica Polonica

authors： Grie P,Koronkiewicz M,Skierski JS

更新日期：2000-01-01 00:00:00

abstract：:A correlation is known to exist in yeast and other organisms between the cellular resistance to stress and the life span. The aim of this study was to examine whether stress treatment does affect the generative life span of yeast cells. Both heat shock (38 degrees C, 30 min) and osmotic stress (0.3 M NaCl, 1 h) applie...

journal_title：Acta biochimica Polonica

authors： Swieciło A,Krawiec Z,Wawryn J,Bartosz G,Biliński T

更新日期：2000-01-01 00:00:00

abstract：:This is a short survey of the adaptive mutation processes that arise in non- or slowly-dividing bacterial cells and includes: (i) bacterial models in which adaptive mutations are studied; (ii) the mutagenic lesions from which these mutations derive; (iii) the influence of DNA repair processes on the spectrum of adapti...

journal_title：Acta biochimica Polonica

authors： Janion C

更新日期：2000-01-01 00:00:00

abstract：:We have studied the ability of yeast DNA polymerases to carry out repair of lesions caused by UV irradiation in Saccharomyces cerevisiae. By the analysis of postirradiation relative molecular mass changes in cellular DNA of different DNA polymerases mutant strains, it was established that mutations in DNA polymerases ...

journal_title：Acta biochimica Polonica

authors： Hałas A,Policińska Z,Baranowska H,Jachymczyk WJ

更新日期：1999-01-01 00:00:00

abstract：:The arginase structural gene (agaA) from Aspergillus nidulans has been cloned and characterised. Depending on the growth conditions of the mycelium, transcripts of this gene have different 5'ends. These differences could result either from the presence of multiple transcription initiation sites or from differential pr...

journal_title：Acta biochimica Polonica

authors： Borsuk P,Dzikowska A,Empel J,Grzelak A,Grześkowiak R,Weglenski P

更新日期：1999-01-01 00:00:00

abstract：:The lifetime distribution calculations were applied to study the influence of configuration of amino-acid residues in positions 2 and 3 on changes in conformation of the peptide chain of cyclic analogues of enkephalins containing a fluorescence energy donor and acceptor in different solvents. In all the solvents studi...

journal_title：Acta biochimica Polonica

authors： Malicka J,Groth M,Czaplewski C,Liwo A,Wiczk W

更新日期：1999-01-01 00:00:00

abstract：:We examined the molecular basis of factor IX deficiency in 53 unrelated Polish patients with hemophilia B. Heteroduplex analysis and direct sequencing of polymerase chain reaction (PCR) products were applied to identify the gene defect. Forty-three different point mutations were detected in the factor IX gene of 47 pa...

journal_title：Acta biochimica Polonica

authors： Wulff K,Bykowska K,Lopaciuk S,Herrmann FH

更新日期：1999-01-01 00:00:00

abstract：:The myosin heavy chain (MHC) was studied by biochemical methods in the slow-twitch (soleus) and two fast-twitch leg muscles of the triiodothyronine treated (hyperthyroid), thyroidectomized (hypothyroid) and euthyroid (control) rats. The changes in the contents of individual MHC isoforms(MHC-1, MHC-2A, MHC-2B and MHC-2...

journal_title：Acta biochimica Polonica

authors： Jakubiec-Puka A,Ciechomska I,Mackiewicz U,Langford J,Chomontowska H

更新日期：1999-01-01 00:00:00

abstract：:In this study we investigated the level of 8-oxo-2'-deoxyguanosine (8-oxodG) in DNA of Cardamine pratensis plants subjected to different growth conditions trying to answer the question whether factors like light and water accessibility or low temperature may have an impact on the total DNA oxidative damage. The level ...

journal_title：Acta biochimica Polonica

authors： Białkowski K,Oliński R

更新日期：1999-01-01 00:00:00

abstract：:Chemical instability of some of the phosphodiester bonds, often observed in large RNAs, visualizes the autocatalytic properties of this class of nucleic acids. Unexpectedly, selective hydrolysis occurs also in short oligoribonucleotides (as short as a tetramer or hexamer). Herein, we describe additional experiments wh...

journal_title：Acta biochimica Polonica

authors： Bibiłło A,Ziomek K,Figlerowicz M,Kierzek R

更新日期：1999-01-01 00:00:00

abstract：:The reaction of nitrite (NO2-) with horseradish peroxidase and lactoperoxidase was studied. Sequential mixing stopped-flow measurements gave the following values for the rate constants of the reaction of nitrite with compounds II (oxoferryl heme intermediates) of horseradish peroxidase and lactoperoxidase at pH 7.0, 1...

journal_title：Acta biochimica Polonica

authors： Gebicka L

更新日期：1999-01-01 00:00:00

abstract：:Since yeast Saccharomyces cerevisiae mutants depleted of the voltage dependent anion selective channel (YVDAC1) are still able to grow on a non-fermentable carbon source, a functional transport system in the outer mitochondrial membrane must exist to support the access of metabolites into mitochondria. It was assumed ...

journal_title：Acta biochimica Polonica

authors： Kmita H,Stobienia O,Michejda J

更新日期：1999-01-01 00:00:00

abstract：:The influence of exogenous inorganic pyrophosphate on the transport of oleanolic acid monoglucoside and monoglucuronide to vacuoles isolated from Calendula officinalis leaf protoplasts was studied. The results indicate that the transport of both monoglycosides is carrier-mediated; however, the transport of the monoglu...

journal_title：Acta biochimica Polonica

authors： Szakiel A,Janiszowska W

更新日期：1998-01-01 00:00:00

abstract：:The paradigm of annexins as phospholipid-binding proteins interacting with membranes in a calcium-dependent manner has been recently questioned in light of observations that some annexin isoforms may behave like membrane integral proteins or remain associated with their target membranes at low, resting, concentrations...

journal_title：Acta biochimica Polonica

authors： Bandorowicz-Pikuła J,Pikuła S

更新日期：1998-01-01 00:00:00

abstract：:The base analogue 6-N-hydroxylaminopurine (HAP) is a potent mutagen in a variety of prokaryotic and eukaryotic organisms. Mutations in the yeast ham1 gene render the cells hypersensitive to the mutagenic effect of HAP. We have found that this gene has homologues in a variety of organisms from bacteria to man. We have ...

journal_title：Acta biochimica Polonica

authors： Kozmin SG,Leroy P,Pavlov YI

更新日期：1998-01-01 00:00:00

abstract：:Public and private EST (Expressed Sequence Tag) programs provide access to a large number of ESTs from a number of plant species, including Arabidopsis, corn, soybean, rice, wheat. In addition to the homology of each EST to genes in GenBank, information about homology to all other ESTs in the data base can be obtained...

journal_title：Acta biochimica Polonica

authors： Rafalski JA,Hanafey M,Miao GH,Ching A,Lee JM,Dolan M,Tingey S

更新日期：1998-01-01 00:00:00

abstract：:The amniotic fluid (AF) was fractionated by dialysis, gel filtration and SDS/PAGE, and submitted to the assay of collagenous constituents. The collagenous character of peptides and proteins of amniotic fluid was confirmed by hydroxyproline (Hyp) assay and treatment with bacterial collagenase followed by electrophoresi...

journal_title：Acta biochimica Polonica

authors： Gogiel T,Bielecki DA,Bańkowski E

更新日期：1998-01-01 00:00:00

abstract：:Intercalative binding of the antitumor drugs ametantrone and mitoxantrone to the dodecamer duplex d(CGCGAGCTCGCG)2 was studied by applying molecular dynamics in water with the GROMOS 87 force field. A number of reasonable binding orientations were tested by short pre-simulations. It was shown that in energetically fav...

journal_title：Acta biochimica Polonica

authors： Mazerski J,Martelli S,Borowski E

更新日期：1998-01-01 00:00:00

abstract：:Two non-conventional analogues of ATP, 3'-deoxyadenosine-2'-triphosphate (3'-d-2'-ATP) and 2'-deoxyadenosine-3'-triphosphate (2'-d-3'-ATP), the syntheses of which are described, were examined as potential phosphate donors for the nucleoside kinases: 2'-deoxycytidine kinase (dCK), cytosolic thymidine kinase (TK1) and m...

journal_title：Acta biochimica Polonica

authors： Krawiec K,Kierdaszuk B,Kalinichenko EN,Mikhailopulo IA,Shugar D

更新日期：1998-01-01 00:00:00

abstract：:Epidemiological studies have revealed a strong correlation between high intake of fruit and vegetables and low incidence of certain cancers. Micronutrients present in these foods are thought to decrease free radical attack on DNA and hence protect against mutations that cause cancer, but the fine details of the causal...

journal_title：Acta biochimica Polonica

authors： Fillion L,Collins A,Southon S

更新日期：1998-01-01 00:00:00

abstract：:Bacteriophage lambda is not able to lysogenise the Escherichia coli rpoA341 mutant. This mutation causes a single amino acid substitution Lys271Glu in the C-terminal domain of the RNA polymerase alpha subunit (alphaCTD). Our previous studies indicated that the impaired lysogenisation of the rpoA341 host is due to a de...

journal_title：Acta biochimica Polonica

authors： Gabig M,Obuchowski M,Ciesielska A,Latała B,Wegrzyn A,Thomas MS,Wegrzyn G

更新日期：1998-01-01 00:00:00

abstract：:Infectious transcripts play a key role in the research on plant viruses at the molecular level. A number of cDNA clones covering the whole genome of the Polish isolate of potato leafroll virus were constructed. Four overlapping clones were selected and assembled using restriction sites. The full copy was positioned be...

journal_title：Acta biochimica Polonica

authors： Sadowy E,Pluta K,Gronenborn B,Hulanicka D

更新日期：1998-01-01 00:00:00

abstract：:Photoreactive gangliosides of high specific radioactivity may prove useful for studies on glycosphingolipid functions. We prepared 4-azidosalicylic acid (ASA) acylated derivatives of GM3, GD3, GM1, and FucGM1 gangliosides (gangliosides-ASA). Gangliosides-ASA were characterized by their TLC mobility, UV spectra, carboh...

journal_title：Acta biochimica Polonica

authors： Pacuszka T,Panasiewicz M

更新日期：1998-01-01 00:00:00

abstract：:An increasing body of evidence suggests that glycolipid domains are present on the plasma membrane surface of mammalian cells and play a key role in signal transduction. We have investigated the modulation of glycolipid-protein interaction consequent to a specific event occurring at the plasma membrane. For this purpo...

journal_title：Acta biochimica Polonica

authors： Masserini M,Pitto M,Ferraretto A,Brunne J,Palestini P

更新日期：1998-01-01 00:00:00

abstract：:A conservative and apparently harmless A176V mutation in intracellular S. cerevisiae L-asparaginase (ScerAI) completely abolishes the enzyme activity. Sequence and structural comparisons with type II bacterial L-asparaginases show that the mutated residue is in a very conservative region and plays a vital role in the ...

journal_title：Acta biochimica Polonica

authors： Bonthron DT,Jaskólski M

更新日期：1997-01-01 00:00:00

abstract：:The results of molecular dynamics simulation of fully hydrated dimyristoylphosphatidylcholine (DMPC) bilayer membrane in the liquid-crystalline phase are presented. They show that the probability of a gauche conformation varies periodically along the chain with only a slight increase towards the end of the chain. Howe...

journal_title：Acta biochimica Polonica

authors： Pasenkiewicz-Gierula M,Róg T

更新日期：1997-01-01 00:00:00

abstract：:Recent reports indicate that minichromosomes and other small genetic entities may occur in the nuclei of uninfected higher plants. They become especially abundant under some special growth conditions and, sometimes, resemble extrachromosomal genes of ciliated protozoa. An example of such gene-sized DNA species was iso...

journal_title：Acta biochimica Polonica

authors： Buchowicz J

更新日期：1997-01-01 00:00:00

abstract：:The properties of red blood cell membranes in patients with chronic renal failure were investigated using electron paramagnetic resonance spectroscopy. Using spin traps, 5,5-dimethylpirroline-1 oxide and N-tert-butyl-alpha-phenylnitrone, we found generation of hydroxyl radicals in the blood of patients with chronic re...

journal_title：Acta biochimica Polonica

authors： Gwoździński K,Janicka M,Brzeszczyńska J,Luciak M

更新日期：1997-01-01 00:00:00

abstract：:The increase in the lipid peroxide level in the serum and liver of female mice after bilateral ovariectomy evidenced antioxidant activities of female hormones. This increase was abolished upon administration of female hormones. Similar increase in the level of lipid peroxide was observed in the serum of women who had ...

journal_title：Acta biochimica Polonica

authors： Yagi K

更新日期：1997-01-01 00:00:00

abstract：:Kainate is a glutamate analog that produces neuronal excitation resulting in seizures within hours following its intraperitoneal injection into adult rats. Then, at 2-3 days after the treatment, neurodegeneration of apoptotic character can be observed in limbic system. As a consequence, plastic reorganization and glia...

journal_title：Acta biochimica Polonica

authors： Kaminska B,Filipkowski RK,Biedermann IW,Konopka D,Nowicka D,Hetman M,Dabrowski M,Gorecki DC,Lukasiuk K,Szklarczyk AW,Kaczmarek L

更新日期：1997-01-01 00:00:00